New Nanospheres to Use in the Determination of Imidan Phosmet and Vantex Pesticides
Özet
In this study, the usability of AChE immobilized nanospheres (NSs) was investigated to identify Imidan Phosmet and Vantex pesticides. Accordingly, (2AEPS-SalBr) coded nanosphere was synthesized in result of condensation of (Tris (2 aminoethyl)amine polymer and 5-bromine-2-hydroxy benzaldehyde. Then, new nanosphere coded 2AEPS-SalBr-Pd was synthesized using the template method. The structures of the synthesized nanospheres were characterized by the FT-IR, GPC, SEM/EDX and elemental analysis. Acetylcholinesterase (AChE) enzyme was immobilized to these nanospheres for the determination of Imidan and Vantex. The optimum conditions of the immobilized-AChE enzyme, the number of reuse, storage stability, and the kinetic parameters were determined. The immobilized AChE optimum pH was 8 for both supports, and the optimum temperature was 50 degrees C and 70 degrees C, respectively. Km and Vmax values were calculated as 1.443 mM and 0.251 mM/min for (2AEPS-SalBr)-AChE (pH 8, at 50 degrees C), and 0.680 mM and 0.157 mM/min for (2AEPS-SalBr-Pd)-AChE (pH 8, at 70 degrees C). Then the analyses of Imidan Phosmet and Vantex pesticides were carried out with immobilized AChE on nanospheres. As a result of the research, it was seen that Imidan Phosmet pesticide can be determined at a lower concentration than Vantex pesticide. [GRAPHICS] .
